Virgin Voyages is dropping the requirement for testing before embarkation.
It comes after the US Centers for Disease Control and Prevention scrapped its Covid-19 monitoring program.
Virgin said it is lifting the testing requirement for Mediterranean cruises on July 24 and from July 27 on US departures.
Scarlet Lady is currently sailing to the Bahamas and Caribbean from Miami and Valiant Lady from Barcelona.
“In response to the discontinuation of the CDC’s Voluntary Program for the Cruise Industry, we’re updating our policies,” it said.
“Passenger and crew wellbeing is the main focus and continue to follow the advice of their expert medical team.”
Virgin Voyages requires 90% of guest capacity to be vaccinated.
All the crew on Virgin ships are fully vaccinated.
Virgin Voyages said it will continue to consider any new recommendations issued by the CDC.